What is the general formula for calculating cfu/ml?


5 colonies/100 ul x 1000 ul/ml x 10^6 dilution = 50x10^6 colonies/ml original = 50 million colonies/ml original.
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1. Perform a preliminary count of each dish once the bacteria have had time to incubate, usually one or two days. You want to count only individual colonies, which should be distinct
cfu/ml = (no. of colonies x dilution factor) / volume inoculated
47 x 5 x 1000000 = 235 x 10^6 per ml.
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