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What Is a Mordant and What Is It Used for?

Answer

A mordant is a chemical substance used in gram staining to intensify a stain. A mordant such as Iodine will form an insoluble complex with the crystal violet or methylene blue and traps the dye in the peptidoglycan layer. Gram staining is usually the 1st step when a bacterial organism is being identified.
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Grams Iodine. Grams Iodine.
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