What Is a Mordant and What Is It Used for?


A mordant is a chemical substance used in gram staining to intensify a stain. A mordant such as Iodine will form an insoluble complex with the crystal violet or methylene blue and traps the dye in the peptidoglycan layer. Gram staining is usually the 1st step when a bacterial organism is being identified.
Q&A Related to "What Is a Mordant and What Is It Used for?"
Mordant is used as fixation to intensifying or deepening the staining action of crystal violet to the organism.
Crystal violet is not fixed and washes off during alcohol treatment, so gram positive cells are stained by saffranine and appear gram negative.
Pink- Gram negative. Purple-Gram positive. :
The positive turn purple and the negative turns red-colored. The positive is purple because the stain is able to pass through the thick peptoglycan wall where as the negative is red
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