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What Is a Mordant and What Is It Used for?

Answer

A mordant is a chemical substance used in gram staining to intensify a stain. A mordant such as Iodine will form an insoluble complex with the crystal violet or methylene blue and traps the dye in the peptidoglycan layer. Gram staining is usually the 1st step when a bacterial organism is being identified.
Q&A Related to "What Is a Mordant and What Is It Used for?"
Grams Iodine Grams Iodine
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A mordant facilitates further interactions between the primary dye
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Crystal violet is not fixed and washes off during alcohol treatment, so gram positive cells are stained by saffranine and appear gram negative.
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1. Place a sample of a bacterial culture on a microscope slide. An inoculation loop can be used to transfer the bacteria to the slide. 2. Use a wooden stick to smear the bacterial
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