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What Is a Mordant and What Is It Used for?

Answer

A mordant is a chemical substance used in gram staining to intensify a stain. A mordant such as Iodine will form an insoluble complex with the crystal violet or methylene blue and traps the dye in the peptidoglycan layer. Gram staining is usually the 1st step when a bacterial organism is being identified.
Q&A Related to "What Is a Mordant and What Is It Used for?"
Grams Iodine Grams Iodine
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A mordant facilitates further interactions between the primary dye
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Crystal violet is not fixed and washes off during alcohol treatment, so gram positive cells are stained by saffranine and appear gram negative.
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Gram staining is important when trying to look at blood or other liquid under a microscope. Staining allows whatever is in the blood to be stained enough to see and stand out.
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Explore this Topic
the purpose of a mordant in the gram stain ' to make the flagella visible. ...
Iodine is often called a "mordant" when used in the gram stain. What its function is, is to form "large" crystals with the Crystal Violet dye ...
Let's review the steps, shall we? 1) Crystal violet - primary stain. Stains all bacteria purple. 2) Iodine - mordant. Complexes with CV to prevents CV from leaving ...
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