What Is a Mordant and What Is It Used for?


A mordant is a chemical substance used in gram staining to intensify a stain. A mordant such as Iodine will form an insoluble complex with the crystal violet or methylene blue and traps the dye in the peptidoglycan layer. Gram staining is usually the 1st step when a bacterial organism is being identified.
Q&A Related to "What Is a Mordant and What Is It Used for?"
the purpose of a mordant in the gram stain ' to make the flagella visible.
A mordant facilitates further interactions between the primary dye
Crystal violet is not fixed and washes off during alcohol treatment, so gram positive cells are stained by saffranine and appear gram negative.
Once a slide of bacterial sample is prepared, crystal violet is used to stain the sample first. At this point both Gram-positive and Gram-negative bacteria will appear purple. Usually
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