What Is a Mordant and What Is It Used for?


A mordant is a chemical substance used in gram staining to intensify a stain. A mordant such as Iodine will form an insoluble complex with the crystal violet or methylene blue and traps the dye in the peptidoglycan layer. Gram staining is usually the 1st step when a bacterial organism is being identified.
Q&A Related to "What Is a Mordant and What Is It Used for?"
Grams Iodine Grams Iodine
A substance used in dyeing to fix the coloring
A mordant is a substance used to set dyes on tissue sections by forming a coordination complex with the dye which then attaches to the fabric or tissue. It may be used for intensifying
Once a slide of bacterial sample is prepared, crystal violet is used to stain the sample first. At this point both Gram-positive and Gram-negative bacteria will appear purple. Usually
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