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Why Is the Size More Accurate in a Negative Stain Than in a Simple Stain?

Answer

The size is more accurate in a negative stain than in simple stain because negative stain only stains the background of the cell; it leaves the object unstained and unchanged. Negative staining is done by using a drop of blank ink called Nigrosin. It is also useful in clearly showing the morphology of a cell.
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since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted
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Negative stain stains the background and leaves the object unstained and unchaged. A simple stain stains the object by permeating it and attaching to its surface, while leaving the
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I suppose that is theoretically true, because in a direct stain a layer of dye molecules attaches to the surface of that which is being stained, while in negative staining nothing
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Since there is no heat fixing or strong cemicals are used the bacteria are less distorted.
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The negative stain and simple stain have differences. The negative stain colors the background because of the capsule. The simple stain only colors the microorganisms ...
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